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Cloning and Expression
DNA Purification
PCR Purification Kits
High Pure PCR Product Purification Kit
High Pure PCR Product Purification Kit from Roche
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Citations:
(32)
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High Pure PCR Product Purification Kit
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Description
For life science research only. Not for use in diagnostic procedures.
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
High Pure PCR Product Purification Kit
Company
Roche
Price
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Get Quote for this Product
Catalog Number
11732668001
Range
The synergistic process requires a minimum fragment length of approximately 100 bp
Quantity
1 kit (50 purifications)
Source
Reaction
Nucleic Acid
DNA Fragment, PCR Product
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Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (32)
(1)
Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites
BMC Microbiology
December 1, 2022
Konrad Egli, Martin Risch, Lorenz Risch, Thomas Bodmer
inhibition.
Purification
of
PCR
products was performed with the
High Pure PCR Product Purification Kit
(order number
11732668001
, product of
Roche
but supplied by Sigma Aldrich) according to the package insert. A maximum of 9.5 μL
(2)
Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans
International Journal of Molecular Sciences
September 22, 2022
Joanna Gawlik, Michal Koper, Albert Bogdanowicz, Piotr Weglenski, Agnieszka Dzikowska
fragments were purified with a
High Pure PCR Product Purification Kit
(
Roche Diagnostics
, cat. no.
11732668001
, Mannheim, Germany). Fragments for
cloning
were amplified using specific primers (Table S1). The Golden
(3)
The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cells
eLife
July 11, 2022
Ávila-González D, Portillo W, Barragán-Álvarez CP, Hernandez-Montes G, Flores-Garza E, Molina-Hernández A, Díaz-Martínez NE, Díaz NF.
isolated using the High Pure FFPET DNA Isolation Kit according to the supplier’s instructions (
Roche
,
11732668001
). The gDNA samples were sent to Germany (Cygenia, https://www.cygenia.com/) to analyze the
DNA methylation
(4)
Cancer-Testis Gene Biomarkers Discovered in Colon Cancer Patients
Genes (Basel)
May 1, 2022
Mikhlid H. Almutairi, Turki M. Alrubie, Abdullah M. Alamri, Bader O. Almutairi, Abdulwahed F. Alrefaei, Maha M. Arafah, Mohammad Alanazi, Abdelhabib Semlali
products were then purified using the
Roche Applied Science
High Pure PCR Product Purification Kit
(
Roche
;
11732668001
, Darmstadt, Germany). Then, 10 ng/μL of DNA in a total volume of 10 μL was placed in a clean 1.5 mL
(5)
锁核苷酸PCR解析植物内生细菌多样性方法
BIO-PROTOCOL
January 1, 2021
镇华 于, Makoto Ikenaga, Masao Sakai, 光华 王
LLC. 1 www.bio-protocol.org/e2003525 4. DOI:10.21769/BioProtoc.2003525
High Pure PCR Product Purification Kit
(
Roche
, Indianapolis IN, USA, catalog number:
11732668001
) 5.
PCR
相关试剂 (Takara RR030A Premix Ex Taq™ Hot Start Version, catalog number: RR030A) 6. LNA 扩增引物和细菌特异性扩增引物
(6)
Molecular Epidemiology of Peste Des Petits Ruminants Cases Associated with Abortion in Sheep and Goat in Marmara Region of Turkey, 2018
The Pakistan Veterinary Journal
December 1, 2020
Zuleyha Pestil
agarose gel electrophoresis
. The amplified
RT-PCR
products were purified using a
High Pure PCR Product Purification Kit
(Lot No.
11732668001
,
Roche
, Germany) and sequenced using an ABI 3100 automatic sequencer. The sequences from both directions
(7)
Identification of genomic insertion and flanking sequences of the transgenic drought-tolerant maize line “SbSNAC1-382” using the single-molecule real-time (SMRT) sequencing method
PLOS ONE
April 10, 2020
Tingru Zeng, Dengfeng Zhang, Yongxiang Li, Chunhui Li, Xuyang Liu, Yunsu Shi, Yanchun Song, Yu Li, Tianyu Wang
(
Roche
, Cat. No.
11585614910
) and purified using the high-purity
PCR product
purification
kit (
Roche
, No.
11732668001
). The DNA blots were pre-hybridized at 42˚C for 1 h in DIG easy hybgranules and then hybridized to denatured
(8)
Increasing the catalytic power of the flavin reductase DszD enzyme using site-directed mutagenesis method in Rhodococcus erythropolis
Journal of Arak University of Medical Sciences
December 30, 2019
Ramin Fallahzadeh, Kasra Esfahani, Abbas Akhavan Sepahi, Nasrin Kamali, Bijan Bambai
،EcoRI، T4 Ligaseکیت استخراج ژل آگارز ( )Cat. No.
11696505001
و کیت تخلیص محصول )Cat. No.
11732668001
)
PCR
از شرکت
Roche
آلمان؛ کیت استخراج )Cat. No. K1014-100) DNAو کیت تخلیص پالسمید ( )Cat.
(9)
Molecular Characterization of Aquaglyceroporine: A Novel Mutation in LmAQP1 from Leishmania major (MRHO/IR/75/ER)
Iran J Parasitol
November 2, 2019
Gilda Eslami, Maryam Ghavami, Ali Reza Moradi, Hamid Nadri, Salman Ahmadian
triplicate.
Sequencing
The amplified fragments were purified using
High Pure PCR Product Purification Kit
(#
11732668001
,
Roche
) and sequenced. The
sequencing
was done triplicate with both forward and reverse primers. Characterization
(10)
Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR
PLOS ONE
June 14, 2018
Sam Kint, Ward De Spiegelaere, Jonas De Kesel, Linos Vandekerckhove, Wim Van Criekinge
kits were also analyzed. The amplicons were purified with the
High Pure PCR Product Purification Kit
(
11732668001
,
Roche
) and sequenced in a MiSeq
sequencing
system (Illumina). The
sequencing
reads were aligned using
(11)
Evolutionary emergence of the
rac3b
/
rfng
/
sgca
regulatory cluster refined mechanisms for hindbrain boundaries formation
Proceedings of the National Academy of Sciences
April 17, 2018
Joaquín Letelier et al.
biological replicates were generated. These libraries were purified with a
PCR Product
Purification kit (No.
11732668001
;
Roche
), their concentrations were measured using the
Qubit dsDNA BR assay kit
(No.
Q32850
;
Molecular Probes
(12)
Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and Visualization
Single Molecule Analysis
January 1, 2018
Yeonee Seol, Keir C. Neuman
Restriction enzyme digestion
BsaI-HF
(R3535,
NEB
) and supplied buffer Products from
PCR reactions
DNA clean-up kit (
11732668001
,
Roche
) 2.1.5
DNA ligation
T4 DNA ligase
(
M1804
,
Promega
) and supplied buffer Products from restriction
(13)
The mitochondrial genomes of the acoelomorph worms Paratomella rubra, Isodiametra pulchra and Archaphanostoma ylvae
Scientific Reports
December 1, 2017
Helen E. Robertson, François Lapraz, Bernhard Egger, Maximilian J. Telford, Philipp H. Schiffer
were purified using the
High Pure PCR Product Purification Kit
(
Roche
Applied Sciences: Product No.
11732668001
) and sent for
sequencing
by Source BioScience Life Sciences. Only amplifications that resulted in one
(14)
Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot Study
Frontiers in Pharmacology
July 27, 2017
Giulia M. Camerino et al.
the pellet of bead were recuperated and purified with
high pure PCR product purification kit
(N.O.
11732668001
Roche
) (Tricarico et al., 2008; Dinardo et al., 2012; Camerino et al., 2013).
Real-time PCR
was performed
(15)
Detection and Genotyping of Human Papillomaviruses from Archival Formalin‐Fixed Tissue Samples
Current Protocols in Microbiology
November 19, 2016
Koenraad Van Doorslaer, Zigui Chen, Alison A. McBride
and vector. Materials PCR clean-up kit (e.g.
High Pure PCR Product Purification Kit
;
Roche
, cat.no.
11732668001
) Agarose Gel
purification
kit (e.g. zymoclean Gel DNA recovery kit; Zymo. cat. no. D4007) 10X ThermoPol
(16)
Single molecule measurements of DNA helicase activity with magnetic tweezers and t-test based step-finding analysis
Methods
August 1, 2016
Yeonee Seol, Marie-Paule Strub, Keir C. Neuman
single-stranded region (Fig. 2b). The digested products were purified using a PCR purification kit (
11732668001
,
Roche
). To complete the 174 bp DNA hairpin, the digestion product was ligated to a 12 bp hairpin DNA
(17)
Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editing
Journal of Assisted Reproduction and Genetics
May 1, 2016
Xiangjin Kang, Wenyin He, Yuling Huang, Qian Yu, Yaoyong Chen, Xingcheng Gao, Xiaofang Sun, Yong Fan
completion of the WGA reaction, the products were purified using the HP
PCR Product
Purification Kit (no.
11732668001
,
Roche
). Purified DNA (1 μl) was run on agarose gels to confirm successful
amplification
, and the remaining
(18)
A single three-dimensional chromatin compartment in amphioxus indicates a stepwise evolution of vertebrate Hox bimodal regulation
Nature Genetics
March 1, 2016
Rafael D Acemel et al.
developmental stage). These libraries were purified with a
High Pure PCR Product Purification Kit
(
Roche
,
11732668001
), their concentrations measured using the Quanti-iTTM PicoGreen dsDNA Assay Kit (
Invitrogen
,
P11496
)
(19)
THE EFFECTS OF CRUDE RECOMBINANT VIRAL PROTEIN VACCINES AGAINST GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) ON HUMPBACK GROUPER (Cromileptes altivelis)
Indonesian Aquaculture Journal
December 31, 2015
Ketut Mahardika, Indah Mastuti
agarose gel 1.5 % in TAE 1x.
PCR product
was purified using a PCR purification kit (
Roche
, Cat. No.
11732668001
) and visualized in agarose gel (1.5 % in TAE 1x) to get a purify and single band. The purificated DNA
(20)
Enhancer of zeste acts as a major developmental regulator of
Ciona intestinalis
embryogenesis
Biology Open
September 15, 2015
Emilie Le Goff, Camille Martinand-Mari, Marianne Martin, Jérôme Feuillard, Yvan Boublik, Nelly Godefroy, Paul Mangeat, Stephen Baghdiguian, Giacomo Cavalli
al., 2010).
PCR
products were sequenced and purified (
High Pure PCR Product Purification Kit
,
Roche
;
11732668001
). Purified
PCR product
(300 ng) was used as template for the DIG
RNA Labeling
Kit (
Roche
;
11277073910
)
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High Pure PCR Product Purification Kit from Roche
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